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Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by <t>ELISA</t> in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by <t>ELISA</t> in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by <t>ELISA</t> in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by <t>ELISA</t> in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by <t>ELISA</t> in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
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Image Search Results


Journal: iScience

Article Title: Cannabinoid receptor type 1 (CB 1 R) inhibits hypothalamic leptin signaling via β-arrestin1 in complex with TC-PTP and STAT3

doi: 10.1016/j.isci.2023.107207

Figure Lengend Snippet:

Article Snippet: mouse/rat specific leptin ELISA kit , B-Bridge International , Cat#K1006-1.

Techniques: Recombinant, Diagnostic Assay, Enzyme-linked Immunosorbent Assay, Produced, Software, Translocation Assay, Imaging, Western Blot

Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by ELISA in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Journal: Science advances

Article Title: An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction.

doi: 10.1126/sciadv.ade4110

Figure Lengend Snippet: Fig. 5. Spironolactone ameliorates adverse cardiac remodeling through hepatic FGF21. (A) Plasma levels of FGF21 tested by ELISA in patients with HP treated without or with spironolactone (Spl). n = 30. (B) Correlation between plasma FGF21 and EF in patients with HP treated with Spl. Pearson correlation test was used. n = 30. (C) Plasma levels of FGF21 tested by ELISA in mice at different time points after MI. ND, normal diet; SD, spironolactone diet. n = 5 to 7. (D) Representative images of echocardiography in mice 1 or 8 weeks after MI. (E) Quantifications of EF, FS, Ds, and Dd based on echocardiography. n = 7 to 9. (F) Representative Masson’s trichrome staining of mouse cardiac sections 1 or 8 weeks after MI. Scale bar, 5 mm. (G) Quantification of scar size in hearts exemplified in (F). n = 10. (H) Representative Picrosirius red staining and WGA staining in noninfarct regions of mouse heart samples 8 weeks after MI. Scale bar, 100 μm. (I) Quantitative analyses of fibrotic area and cardiomyocyte size. n = 7. (J) HW/BW, HW/TL, and LW/BW of mice 8 weeks after MI. n = 8. (K) qRT-PCR analysis of ANP and BNP gene expression in mouse heart samples 8 weeks after MI. n = 7. Data are represented as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: FGF21 concentrations were detected using a mouse-specific ELISA kit (MF2100, R&D Systems, Minneapolis, MN) or humanspecific ELISA kit (DF2100, R&D Systems) according to the manufacturer’s instructions.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Staining, Quantitative RT-PCR, Gene Expression